Journal: Frontiers in Oncology

Article Title: Cytokine-Mediated STAT3 Transcription Supports ATGL/CGI-58-Dependent Adipocyte Lipolysis in Cancer Cachexia

doi: 10.3389/fonc.2022.841758

Figure Lengend Snippet: Key Resources.

Article Snippet: Mouse monoclonal anti-CGI-58 , Novus Biologicals , Cat# H00051099-M01.

Techniques: Recombinant, Protease Inhibitor, Transfection, Saline, Bicinchoninic Acid Protein Assay, Solvent

Journal: Frontiers in Oncology

Article Title: Cytokine-Mediated STAT3 Transcription Supports ATGL/CGI-58-Dependent Adipocyte Lipolysis in Cancer Cachexia

doi: 10.3389/fonc.2022.841758

Figure Lengend Snippet: Key Resources.

Article Snippet: Mouse monoclonal anti-CGI-58 , Novus Biologicals , Cat# H00051099-M01.

Techniques: Recombinant, Protease Inhibitor, Transfection, Saline, Bicinchoninic Acid Protein Assay, Solvent

Journal: Domestic animal endocrinology

Article Title: Adipose triglyceride lipase protein abundance and translocation to the lipid droplet increase during leptin-induced lipolysis in bovine adipocytes.

doi: 10.1016/j.domaniend.2017.06.001

Figure Lengend Snippet: Fig. 1. Demonstration of image assessment. The figure illustrates how translocation was measured for a lipid droplet using the RGB Profiles Plot plug in ImageJ [36]. The white lines represent where lines would have been drawn in ImageJ. The upper inset box is a representation of the data produced by RGB Profiles Plot plug in ImageJ. The intensity values along each pixel in the line were exported, averaged at either 0 and 0.89 mm for the average fluorescence at the lipid droplet, or 2.70 and 3.30 mm for the average fluorescence in the cytosol, and utilized for statistical analysis. Green represents the lipid droplet, blue and red represent ATGL and ABHD5, respectively. ABHD5, a/b hydroxylase domain containing 5; ATGL, adipose triglyceride lipase. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Antibody Company Catalog number PSTAT3 Cell Signaling 9145 HSL Cell Signaling 8719 ATGL Cell Signaling 2138 ABHD5 Santa Cruz Biotechnology Sc-102285 G0S2 Novus Biologicals 4137 Abbreviations: ABHD5, a/b hydroxylase domain containing 5; ATGL, adipose trigly 2; HSL, hormone sensitive lipase; PSTAT3, phosphorylated signal transducer and a All secondary antibodies were purchased from Jackson Immunoresearch in W ruminants adipose tissue is limited to a single study in sheep that did not observe the activation of STAT3 or STAT5 [29].

Techniques: Translocation Assay, Produced

Journal: Domestic animal endocrinology

Article Title: Adipose triglyceride lipase protein abundance and translocation to the lipid droplet increase during leptin-induced lipolysis in bovine adipocytes.

doi: 10.1016/j.domaniend.2017.06.001

Figure Lengend Snippet: Fig. 8. Time course of adipose triglyceride lipase (ATGL) and a/b hydroxylase domain containing 5 (ABHD5) translocation. N ¼ 12–15 lipid droplets from 2 cultures per treatment. (A) Representative images of SVC-derived adipocytes treated with 100 ng/mL of leptin over the course of 2 h. Time of exposure is listed on the left hand side. In the merged image, blue represents ATGL, green represents bodipy, and red represents ABHD5. (B) Average translocation of ATGL to the lipid droplet for both images that contained ABHD5 and G0S2 SEM. The white line in the lower right hand image is 10 mm relative to the view. Differences in subscripts were given when P < 0.05 and indicate pairwise differences of P < 0.05. SEM, standard error of the mean. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Antibody Company Catalog number PSTAT3 Cell Signaling 9145 HSL Cell Signaling 8719 ATGL Cell Signaling 2138 ABHD5 Santa Cruz Biotechnology Sc-102285 G0S2 Novus Biologicals 4137 Abbreviations: ABHD5, a/b hydroxylase domain containing 5; ATGL, adipose trigly 2; HSL, hormone sensitive lipase; PSTAT3, phosphorylated signal transducer and a All secondary antibodies were purchased from Jackson Immunoresearch in W ruminants adipose tissue is limited to a single study in sheep that did not observe the activation of STAT3 or STAT5 [29].

Techniques: Translocation Assay, Derivative Assay